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Liquid Biopsy Technology in Cancer Research and Metastatic Signaling

University of Patras
(4.67/5) - 3 reviews

Associate Professor Galatea Kallergi is a leading researcher in the field of cancer biology, with a specialized focus on liquid biopsy, circulating tumor cells (CTCs), and metastatic signaling. Based at the Department of Biology, University of Patras, Greece, she brings over two decades of experience in translational oncology. Her pioneering work has contributed significantly to understanding the molecular and phenotypic characteristics of CTCs and their role in cancer progression, drug resistance, and immune evasion. With an extensive publication record, international collaborations, and expertise in both fundamental and applied cancer research, Professor Kallergi is committed to mentoring the next generation of scientists in precision oncology.

This 6-week onsite fellowship, hosted at the University of Patras by Associate Professor Galatea Kallergi, offers a focused exploration of liquid biopsy technologies in cancer research, with a strong emphasis on Circulating Tumor Cells (CTCs) and Extracellular Vesicles (EVs). Fellows will gain in-depth exposure to cutting-edge techniques for isolation, phenotypic characterization, molecular profiling, and drug response testing using patient-derived samples. The program integrates theoretical grounding with practical lab training and is ideal for those interested in translational oncology, metastasis research, and the development of precision medicine strategies.

Main Areas of Interest

verview of Liquid Biopsy (LB)

Types of analytes:

Circulating Tumor Cells (CTCs)
Extracellular Vesicles (EVs)

1. Characteristics and properties of CTCs and EVs

2. Role of CTCs and EVs in cancer progression, metastasis, and disease monitoring

Liquid Biopsy Applications in Cancer Research

Isolation and molecular characterization of CTCs and EVs for disease monitoring
Integration of LB techniques to inform treatment decisions and enhance patient outcomes
Advancing precision oncology through the use of liquid biopsy in personalized medicine

Specialties

Liquid Biopsy
Circulating Tumor Cells (CTCs)
Extracellular Vesicles (EVs)
Cancer Metastasis
Precision Oncology

Specialties

Oncology

Cancer

Biochemistry

Physiology

Techniques You Will Learn

1. General Knowledge of Liquid Biopsy

Isolation and Enrichment of CTCs and EVs:Learn techniques for isolating CTCs and EVs from blood samples using size-based filtration, microfluidic platforms, and density gradient methods.
Immunofluorescence (IF) Staining: Gain experience in phenotypic characterization using established immunofluorescence staining panels.

2. Molecular and Functional Analysis

Microscopy and Imaging Techniques:Training in high-resolution imaging to visualize CTC morphology and EV structure in detail.
Molecular Analysis: Hands-on exposure to immunostaining, quantitative PCR (qPCR), and immunoblotting for studying the molecular profiles of CTCs and EVs.

3. Technology Development

Microfluidic Platforms: Practical experience with microfluidic systems for enhanced cancer cell culture and analysis.
Multi-Step Assay Development:Learn how to design sequential assays that allow analysis of multiple features in CTCs and EVs from a single patient sample to improve diagnostic accuracy.

4. Drug Response and Therapeutic Investigation

Functional Assays for Investigating Drug Impact: Training in evaluating anti-cancer drug effects on cell viability, apoptosis, and invasion potential in cancer cell lines and patient-derived samples.
Translation to Cultured CTCs: Development of experimental protocols to adapt drug response studies specifically to cultured CTCs derived from patient blood samples.
response studies for cultured CTCs derived from patient samples.

Curriculum

Sample 2-Week Curriculum

Please note: The curriculum below is a sample structure. Specific activities may vary based on the applicant’s background, ongoing lab projects, and availability of resources.

Week 1: Foundations in Liquid Biopsy and Laboratory Techniques

Day 1–2:

Orientation to the laboratory environment
Introduction to liquid biopsy concepts, including CTCs and EVs
Overview of biospecimen handling, safety protocols, and patient sample logistics

Day 3–4:

Training in blood sample processing and enrichment techniques for CTCs and EVs
Hands-on practice with size-based filtration and density gradient centrifugation
Introduction to microfluidic platforms for CTC/EV isolation

Day 5:

Basics of immunofluorescence staining for CTC identification
Use of epithelial and mesenchymal markers in cancer cell profiling

Week 2: Advanced Applications and Translational Techniques

Day 6–7:

High-resolution imaging of CTCs and EVs
Interpretation of microscopy results and digital documentation

Day 8:

Molecular analysis of CTCs/EVs (e.g., qPCR, immunoblotting)
Discussion on data reproducibility and validation

Day 9:

Functional assays to evaluate cell viability and drug responsiveness
Setting up in vitro experiments using patient-derived cells

Day 10:

Summary of findings and potential research directions
Feedback session and discussion of future collaborations/publication potential

Important Note:

This sample curriculum is intended as a general guide. The exact schedule and modules may be adjusted depending on the applicant’s educational level, research focus, and ongoing projects in the lab. Personalized learning goals can be accommodated upon discussion with the host mentor.

How much it costs the host mentor to teach you (Trialect curated)

hese are heavily discounted training programs: Please see below the original costs

1. Sample Preparation & Isolation

Blood collection tubes (EDTA, Streck, or similar): $5–10 per tube × ~20–30 = $100–300

Ficoll/gradient media: ~$150–250 per 500 mL (enough for ~50 preps)

Filtration devices/microfluidic cartridges: $50–100 each × ~10–15 = $500–1,500

Subtotal: $800–2,000

2. Immunofluorescence & Staining

Primary antibodies (e.g., EpCAM, CK, CD45, EV markers): $300–500 each, at least 3–4 = $1,200–2,000

Secondary antibodies (fluorophore conjugated): $250–400 each × 2–3 = $500–1,200

Mounting media, DAPI, coverslips, slides, blocking reagents: $200–400

Subtotal: $2,000–3,500

3. Molecular Assays

qPCR reagents (SYBR/Probe mixes, primers): ~$1,000 for full 2 weeks

Western blot reagents (gels, buffers, antibodies, membranes): $1,000–1,500

RNA/DNA extraction kits: $300–500

Subtotal: $2,000–3,000

4. Drug Response Assays

Cell culture media, serum, supplements: $500–800

Drugs (commercial anti-cancer compounds or small panels): $500–1,000 (small-scale testing)

Apoptosis/viability kits (Annexin V, MTT/XTT, etc.): $300–500

Subtotal: $1,300–2,300

5. Microfluidics & Technology Development

Prototype chips/cartridges: $100–200 each × 10 = $1,000–2,000

Tubing, connectors, PDMS reagents (if custom casting): $500–700

Subtotal: $1,500–2,500

Estimated Total for 2 Weeks

$7,600 – $13,300
(assuming 1–2 fellows, moderate repetition of assays, not running full cohorts daily)

Accommodation

Accommodation

The participant will arrange their own accommodations close location to the institute.
You may also consider options on Airbnb.com for short-term stays.

Fees and Program Logistics

This program has the following durations available:

Duration	Fee
2 weeks	$1,063.00

Who Can Apply

Undergraduate, postgraduate, postdoctoral candidates are welcome. Faculty can also visit.

Host Details

Host Name: Dr.Galatea Kallergi

Affiliation: University of Patras

Address: University of Patras, 26504 Rio, Greece

Website URL: https://biology.upatras.gr/personel/kallergi/

Disclaimer:It is mandatory that all applicants carry workplace liability insurance, e.g., https://www.protrip-world-liability.com (Erasmus students use this package and typically costs around 5 € per month - please check) in addition to health insurance when you join any of the onsite Trialect partnered fellowships.

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Onsite/On-Campus Program

Fellowship - Basic/Translational/Clinical Research Program

Greece

Application Review Deadline:

Feb 15th, 2026

Apply Now

Featured Reviews

4.67

3 reviews

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-- The Liquid Biopsy Technology in Cancer Research and Metastatic Signaling program is nothing short of amazing! I'd rate it a solid 5/5 for the unparalleled learning experience it offers. The mentors are highly knowledgeable and supportive, and the hands-on clinical research experience is invaluable. I also appreciate the networking opportunities and the chance to publish my own research articles. -- -- I am so grateful for the opportunity to be a part of the Liquid Biopsy Technology in Cancer Research and Metastatic Signaling program. It is a solid 5/5 for me. The exposure to clinical trials, pharmacovigilance, and research methodologies has been incredibly valuable. The support and guidance from the host mentor and team are unparalleled. Plus, the program's commitment to fostering long-term professional connections is commendable. -- -- I am very impressed with the Liquid Biopsy Technology in Cancer Research and Metastatic Signaling program. A definite 5/5! The program offers a great opportunity to immerse in French culture, practice language skills, and explore new research environments. Furthermore, the hands-on clinical research experience is top-notch. --

🌟 Overall Sentiment: Highly Positive

Most participants rated the Liquid Biopsy Technology in Cancer Research and Metastatic Signaling program 5/5, praising its high-quality mentorship, hands-on clinical research experience, and networking opportunities. The exposure to clinical trials, pharmacovigilance, and research methodologies was highly valued by the participants. In addition, the chance to immerse in French culture, practice language skills, and explore new research environments was appreciated.

Key Strengths

🧑‍🔬 Hands-on Clinical Research Experience – Participants benefited from valuable exposure to clinical trials, pharmacovigilance, and research methodologies.
👨‍🏫 Supportive & Knowledgeable Mentors – The host mentor and team were praised for their expertise and guidance.
🤝 Collaboration & Networking – Participants appreciated the opportunities to publish research articles and create long-term professional connections.
🌍 Cultural & Professional Exposure – The program allowed participants to immerse in French culture, practice language skills, and explore new research environments.

Samar El-Achy 2 weeks ago

(5/5)

Some of the techniques planned by Professor Kalergi’s team were dependent on the availability of freshly collected patient samples, which occurred irregularly. As a result, a few of the intended procedures could not be performed during the training period due to the limited availability of fresh material. To enhance future training experiences, it may be beneficial to develop a schedule that includes pre-prepared or archived samples. This would ensure that a wider range of experimental techniques can be demonstrated and practiced consistently, regardless of the timing of new sample collection.

The visa process itself was smooth and well-organized. The only challenge I encountered was securing an appointment at the Greek Embassy in Egypt, as appointment slots were completely closed during June and July and only reopened in August. This caused a delay in my travel plans, postponing my intended departure from September to October. Nevertheless, once the appointment was scheduled, the visa procedures proceeded efficiently and without further difficulty.

Traveling from Cairo to Athens was straightforward, followed by a bus journey to Patras, which was convenient and easy to manage. For accommodation, Professor Kalergi kindly recommended the “Moxy” Hotel by the Marina in Patras—a comfortable, safe, and well-located option. Upon contacting the hotel administration prior to booking, I explained that I was visiting the University of Patras for an extended training period, and they generously offered me a special discounted rate.

Professor Kalergi and her team were exceptionally hospitable, even offering daily transportation to and from the laboratory. Their kindness and support greatly facilitated my stay and contributed to a smooth and enjoyable overall experience.

One of the key strengths of this fellowship was the clear and productive exchange of knowledge between myself and the team at Professor Galatea Kalergi’s laboratory. The group demonstrated deep expertise in circulating tumor cells (CTCs) within the context of liquid biopsies, with each researcher exploring the topic from a distinct perspective. This structure allowed me to observe and understand the workflow from multiple angles, gaining a comprehensive overview of the field.

At the same time, my own background in the clinical management of cancer contributed meaningfully to our discussions, helping to clarify several practical and translational questions raised by the team. The integration of our respective expertise—particularly in cell culture, immunofluorescence staining, and confocal microscopy—created a highly dynamic and enriching learning environment.

Moreover, the laboratory and the Department of Biology at the University of Patras were exceptionally welcoming and professional. The atmosphere of hospitality, generosity, and collegiality greatly enhanced the overall experience and stands out as one of the most valuable aspects of this fellowship.

Samar El-Achy 2 weeks ago

(5/5)

MICHELE SANTILLO 3 months ago

(4/5)

I really had a meaningful experience with this program and fellowship;
-The things that stood out in my opinion were the professionalism of the group I found myself in and their welcome, cutting-edge techniques and equipment and excellent availability;

- Overall rating: 4/5 (given only by the impossibility of extending the period and the high price but I still understand the necessary expenses)